Prof. Dr. Thomas Kuner

Curriculum vitae


MD 1998, University of Heidelberg
1999-2000, Postdoctoral work at Duke University Medical Center, Durham, and Marine Biological Laboratory, Woods Hole, USA
Since 2000, Group leader at the Max-Planck-Institute for Medical Research, Heidelberg
2003, Habilitation in Physiology, University of Heidelberg
2006, Professor of Anatomy and Cell Biology, University of Heidelberg
Current position: Professor, Director, Dept. of Functional Neuroanatomy
(Full CV)


A. Originalarbeiten


1.   Raditsch, M., Ruppersberg, J. P., Kuner, T., Gunther, W., Schoepfer, R., Seeburg, P. H., Jahn, W., and Witzemann, V. (1993). Subunit-specific block of cloned NMDA receptors by argiotoxin636. FEBS Lett 324, 63-6.


2.   Kuner, T., Schoepfer, R., and Korpi, E. R. (1993). Ethanol inhibits glutamate-induced currents in heteromeric NMDA receptor subtypes. Neuroreport 5, 297-300.


3.   Korpi, E. R., Kuner, T., Kristo, P., Kohler, M., Herb, A., Luddens, H., and Seeburg, P. H. (1994). Small N-terminal deletion by splicing in cerebellar alpha 6 subunit abolishes GABAA receptor function. J Neurochem 63, 1167-70.


4.   Lomeli, H., Mosbacher, J., Melcher, T., Hoger, T., Geiger, J. R., Kuner, T., Monyer, H., Higuchi, M., Bach, A., and Seeburg, P. H. (1994). Control of kinetic properties of AMPA receptor channels by nuclear RNA editing. Science 266, 1709-13.


5.   Korpi, E. R., Kuner, T., Seeburg, P. H., and Luddens, H. (1995). Selective antagonist for the cerebellar granule cell-specific gamma-aminobutyric acid type A receptor. Mol Pharmacol 47, 283-9.


6.   Wollmuth, L. P.*, Kuner, T.*, Seeburg, P. H., and Sakmann, B. (1996). Differential contribution of the NR1- and NR2A-subunits to the selectivity filter of recombinant NMDA receptor channels. J. Physiol. (Lond.) 491, 779-797.


7.   Kuner, T. #, and Schoepfer, R. (1996). Multiple Structural Elements Determine Subunit-Specificity of Mg2+ Block in NMDA Receptor Channels. J. Neuroscience 16, 3549-3558.


8.   Kuner, T. #, Wollmuth, L. P., Karlin, A., Seeburg, P. H., and Sakmann, B. (1996). Structure of the NMDA receptor channel M2 segment inferred from the accessibility of substituted cysteines. Neuron 17, 343-352.


9.   Wollmuth, L. P.*, Kuner, T.*, and Sakmann, B. (1998). Interaction of Internal Mg2+ with Structural Determinants of the Selectivity Filter in Recombinant NMDAR Channels.  J. Physiol. (Lond.) 506, 33-52.


10. Wollmuth, L. P.*, Kuner, T.*, and Sakmann, B. (1998). Adjacent Asparagines in the NMDAR NR2 Subunit Underlie the Voltage Dependent Block by External Mg2+. J. Physiol. (Lond.) 506, 13-32.


11. Saul, B.*, Kuner, T.*, Sobetzko, D., Brune, W., Hanefeld, F., Meinck, H-M., and Becker, C-M. (1999). Novel GLRA1 missense mutation (P250T) in dominant hyperekplexia defines an intracellular determinant of glycine receptor channel gating.  J. Neuroscience 19, 869-877.


12. Wollmuth, L. P., Kuner, T., Jatzke, C., Seeburg, P. H., Heintz, N., and Zuo, J. (2000). Lurcher identifies delta2 as a Ca2+ permable AMPA/Kainate receptor–like channel that is potentiated by Ca2+. J. Neuroscience 20, 5973-5980.


13. Beck, C., Wollmuth, L. P., Seeburg, P. H., Sakmann, B., and Kuner, T.# (1999). NMDA receptor channel segments forming the extracellular vestibule inferred from the accessbility of substituted cysteines. Neuron 22, 559-570.


14. Kuner, T. and Augustine, G. J. (2000). A genetically encoded ratiometric indicator for chloride: capturing chloride transients in hippocampal neurons. Neuron 27, 447-459.


15. Kuner, T. #, Beck, C., Sakmann, B., Seeburg, P. H. (2001). Channel-lining residues of the AMPA receptor M2 segment: structural environment of the Q/R site and identification of the selectivity filter. J. Neuroscience 21:4162-4172.


16. Watanabe, J., Beck, C., Kuner, T., Premkumar, L., and Wollmuth, L. P. (2002). DRPEER: A Motif in the Extracellular Vestibule Conferring High Ca2+ Flux Rates in NMDA Receptor Channels. J. Neuroscience 22:10209–10216.


17. Dreyer, J., Schleicher, M., Tappe, A., Schilling, K., Kuner, T., Kusumawidijaja, G., Muller-Esterl, W., Oess, S., Kuner, R. (2004). Nitric Oxide Synthase (NOS)-Interacting Protein Interacts with Neuronal NOS and Regulates Its Distribution and Activity. J Neurosci. 24(46): 10454-10465.


18. Maurer, M.H., Grunewald, S., Gassler, N., Rossner, M., Propst, F., Wurz, R., Weber, D., Kuner, T., Kuschinsky, W., Schneider, A. (2004). Cloning of a novel neuronally expressed orphan G-protein-coupled receptor. J Neurochem. 91(4): 1007-17.


19. Wimmer, V.C., Nevian, T., and Kuner, T.# (2004). Targeted in vivo expression of proteins in the calyx of Held. Pflugers Archiv/European Journal of Physiology 449, 319-333.


20. Abraham, N.M., Spors, H., Carleton, A., Margrie, T., Kuner, T., Schaefer, A.T. (2004). Maintaining accuracy at the expense of speed: stimulus similarity defines odor discrimination time in mice. Neuron 44, 865-876.


21. Haverkamp, S., Wässle, H., Duebel, J., Kuner, T., Augustine, G.J., Feng, G., Euler, T. (2005). The primordial blue cone color system of the mouse retina. J. Neurosci 25, 5438-5445.


22. Dübel, J., Haverkamp, S., Schleich, W., Augustine, G.J., Feng, G., Kuner, T. #, Euler, T.# (2006). Two-photon imaging reveals somato-dendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Neuron 49, 81-94.


23. Wimmer, V.C., Horstmann, H., Groh, A., Kuner, T. # (2006). Donut-like topology of synaptic vesicles with a central cluster of mitochondria wrapped into membrane protrusions: a novel structure-function module in the adult calyx of Held. J Neurosci 26, 109-116.


24. Pond, B.B., Berglund, K., Kuner, T., Feng, G., Augustine, G.J., Schwartz-Bloom, R.D. (2006). The chloride transporter Na(+)-K(+)-Cl- cotransporter isoform-1 contributes to intracellular chloride increases after in vitro ischemia. J Neurosci 26:1396-1406.


25. Nair, D.K., Jose, M., Kuner, T., Zuschratter, W., Hartig, R. (2006). FRET-FLIM at nanometer spectral resolution from living cells. Optics Express 14:12217-12229.


26. Berglund, K., Schleich, W., Krieger, P., Loo, L.S., Wang, D., Cant, N.B., Feng, G., Augustine, G.J., Kuner, T.# (2006). Imaging synaptic inhibition in mice expressing the chloride indicator, Clomeleon. Brain Cell Biology 35, 207-235.


27. Tappe, A., Agarwal, N., Katona, I., Rubino, T., Swiercz, J., Mackie, K., Monyer, H., Whistler, J. Kuner, T., Kuner, R. (2007). A molecular basis of analgesic tolerance to cannabinoids. J Neurosci. 27, 4165-4177.


28. Krieger, P., Kuner, T., Sakmann, B. (2007). Synaptic connections between layer 5B pyramidal neurons in mouse somatosensory cortex are independent of apical dendrite bundling. J. Neurosci., 27:11473-11482.


29. Kremer, T., Kempf, C., Wittenmeyer, N., Nawrotzki, R., Kuner, T., Kirsch, J., Dresbach, T. (2007). Mover is a novel vertebrate-specific presynaptic protein with differential distribution at subsets of CNS synapses. FEBS Letters, 581:4727-4733.


30. Kuner, T.#, Li, Y., Gee, K. R., Bonewald, L. F., Augustine, G. J. (2008). Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. PNAS 108, 347-352.


31. Groh, A., de Kock, C.P.J., Wimmer, V.C., Sakmann, B., Kuner, T.# (2008). Driver or coincidence detector: modal switch of a corticothalamic giant synapse controlled by spontaneous activity and short-term depression. J Neurosci. 28, 9652-9663.


32. Berglund, K., Schleich, W., Hong, W., Feng, G., Hall, W.C., Kuner, T., Augustine, G.J. (2008). Imaging synaptic inhibition throughout the brain via genetically targeted Clomeleon. Brain Cell Biology 36:101-118.


33. Wittenmayer, N., Körber, C., Liu, H., Kremer, T., Varoqueaux, F., Chapman, E.R., Brose, N., Kuner, T., Dresbach, T. (2009). Postsynaptic Neuroligin1 regulates presynaptic maturation. PNAS 106, 13564-13569.


34. Glykys, J., Dzhala, V.I., Kuchibhotla, K.V, Feng, G., Kuner, T., Augustine, G., Bacskai, B.J., Staley, K.J. (2009). Differences in cortical versus subcortical GABAergic signaling: a candidate mechanism of electroclinical dissociation of neonatal seizures. Neuron 63, 657-672.


35. Dondzillo, A., Sätzler, K., Horstmann, H., Altrock, W.A., Gundelfinger, E.D., Kuner, T.  (2010). Targeted three-dimensional immunohistochemistry reveals localization of presynaptic proteins bassoon and piccolo in the rat calyx of Held before and after the onset of hearing. J. Comp. Neurol. 518, 1008-1029.


36. Abraham, N.M., Egger, V., Shimshek, D.R., Renden, R., Fukunaga,. I., Sprengel, R., Seeburg, P.H., Klugmann, M., Margrie, T.W., Schaefer, A.T., Kuner, T. (2010). Synaptic inhibition in the olfactory bulb accelerates odor discrimination in mice. Neuron 65, 399-411.


37. Wimmer, V.C., Bruno, R.M., de Kock, C.P.J., Kuner, T., Sakmann B (2010). Dimensions of an innervation column and architecture of VPM- and POm-projections in rat vibrissal cortex. Cerebral Cortex 20: 2265-2276.


38. Dzhala, V., Kuchibhotla, K., Glykys, J., Kahle, K., Swiercz, W., Feng, G., Kuner, T., Augustine, G.J., Bacskai, B., Staley, K. (2010). Progressive NKCC1-dependent neuronal chloride accumulation during neonatal seizures. Journal of Neuroscience 30, 11745-761.


39. Schwenger, D. and Kuner, T. (2010). Acute genetic perturbation of exocyst function in the rat calyx of Held impedes structural maturation, but spares synaptic transmission. European Journal of Neurosciences, 32:974-984.


40. Wimmer, V.C, Broser, P.J., Kuner, T., Bruno, R.M. (2010). Deprivation-induced anatomical plasticity of thalamocortical axons in adults. Journal of Comparative Neurology, 518:4629–4648.


41. Denker, A., Bethani, I., Kröhnert, K., Körber, C., Horstmann, H., Wilhelm, B.G., Barysch, S.V., Kuner, T., Neher, E., Rizzoli, S.O. (2011). A small pool of vesicles maintains synaptic activity in vivo. PNAS 108: 17177-17182.


42. Luo C., Gangadharan V., Bali K., Xie R.G., Agarwal N., Kurejova M., Tappe-Theodor A., Tegeder I., Feil S., Lewin G., Polgar E., Todd A.J., Schlossmann J., Hofmann F., Liu D.L., Hu S.J., Feil R., Kuner T., Kuner R. (2012). Presynaptically Localized Cyclic GMP-Dependent Protein Kinase 1 Is a Key Determinant of Spinal Synaptic Potentiation and Pain Hypersensitivity. PLoS Biol 10(3): e1001283. doi:10.1371/journal.pbio.1001283.


43. Horstmann, H., Körber, C., Sätzler, K., Aydin, D., Kuner, T. (2012). Serial Section Scanning Electron Microscopy (S3EM) on Silicon Wafers for Ultrastructural Volume Imaging of Cells and Tissues. PLoS One 7(4): e35172. doi:10.1371/journal.pone.0035172.


44. Orenbuch, A., Shulman, Y., Lipstein, N., Bechar, A., Lavy, Y., Brumer, E., Vasileva, M., Kahn, J., Kuner, T., Gitler, D. (2012). Endocytosis is essential for synapsin-dependent mobilization of reserve pool vesicles. Journal of Neurochemistry 120:248-258.


45. Nanguneri, S., Flottmann, B., Horstmann, H., Heilemann, M., Kuner, T. (2012). Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples. PloS one, 7(5), e38098. doi:10.1371/journal.pone.0038098


46. Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J. (2012). Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons. Mol Cell Neurosci 50: 250-259.


47. Sheng, J., He, L., Zheng, H., Xue, L., Luo, F., Shin, W., Sun, T., Kuner, T., Yue, D.T., Wu, L.-G. (2012). Calcium-channel number critically influences synaptic strength and plasticity at the active zone. Nature Neuroscience 15:998-1006.


48. Vasileva, M., Horstmann, H., Geumann, C., Gitler, D., Kuner, T. (2012). Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission. European Journal of Neuroscience, 36:3005-3020.


49. Körber, C., Horstmann, H., Sätzler, K., Kuner, T. (2012). Endocytic structures and synaptic vesicle recycling at a central synapse in awake rats. Traffic, 13:1601-11.


50. Kempf, C., Staudt, T., Bingen, P., Horstmann, H., Engelhardt, J., Hell, S. W., & Kuner, T. (2013). Tissue Multicolor STED Nanoscopy of Presynaptic Proteins in the Calyx of Held. PloS one, 8(4), e62893. doi:10.1371/journal.pone.0062893


51. Horstmann, H., Vasileva, M., & Kuner, T. (2013). Photooxidation-Guided Ultrastructural Identification and Analysis of Cells in Neuronal Tissue Labeled with Green Fluorescent Protein. PloS One, 8(5), e64764. doi:10.1371/journal.pone.0064764


52. Gangadharan, V., Selvaraj, D., Kurejova, M., Njoo, C., Gritsch, S., Škoricová, D., Horstmann, H., Offermanns, S., Brown, A.J., Kuner, T., Tappe-Theodor, A. and Kuner, R. (2013). A novel biological role for the phospholipid lysophosphatidylinositol in nociceptive sensitization via activation of diverse G-protein signalling pathways in sensory nerves in vivo., Pain 154(12), 2801–2812. doi:10.1016/j.pain.2013.08.019


53. Vasileva, M., Renden, R., Horstmann, H., Gitler, D. and Kuner, T. (2013). Overexpression of synapsin Ia in the rat calyx of Held accelerates short-term plasticity and decreases synaptic vesicle volume and active zone area. Frontiers in Cellular Neurosciences 7, 270. doi:10.3389/fncel.2013.00270


54. Körber, C., Dondzillo, A., Eisenhardt, G., Herrmannsdörfer, F., Wafzig, O., & Kuner, T. (2014). Gene expression profile during functional maturation of a central mammalian synapse. European Journal of Neuroscience, n/a–n/a. doi:10.1111/ejn.12661


55. Nanguneri, S., Flottmann, B., Herrmansdörffer, F., Kuner, T., and Heilemann, M. (2014). Single-Molecule Super-Resolution Imaging by Tryptophan-Quenching-Induced Photoswitching of Phalloidin-Fluorophore Conjugates. Microscopy Research and Technique, 510-6. Doi:

56. Mark MD, Krause M, Boele HJ, Kruse W, Pollok S, Kuner T, Dalkara D, Koekkoek S, De Zeeuw CI, Herlitze S. (2015). Spinocerebellar Ataxia Type 6 Protein Aggregates Cause Deficits in Motor Learning and Cerebellar Plasticity. J Neurosci. 2015 Jun 10;35(23):8882-95.


57. Körber, C., Horstmann, H., Venkatamarani, V., Herrmannsdörfer, Kremer, T., Kaiser, M., Schwenger, D.B., Ahmed, S., Dean, C., Dresbach, T. & Kuner, T. (2015). Modulation of Presynaptic Release Probability by the Vertebrate-Specific Protein Mover. Neuron 87, 521-533.


58. Seol, M. and Kuner, T. (2015). Ionotropic glutamate receptor GluA4 and T-type calcium channel Cav3.1 subunits control key aspects of synaptic transmission at the mouse L5B-POm giant synapse. European Journal of Neuroscience 42, 3033-3044.


59. Osswald M, Jung E, Sahm F, Solecki G, Venkataramani V, Blaes J, Weil S, Horstmann H, Wiestler B, Syed M, Huang L, Ratliff M, Karimian Jazi K, Kurz FT, Schmenger T, Lemke D, Gömmel M, Pauli M, Liao Y, Häring P, Pusch S, Herl V, Steinhäuser C, Krunic D, Jarahian M, Miletic H, Berghoff AS, Griesbeck O, Kalamakis G, Garaschuk O, Preusser M, Weiss S, Liu H, Heiland S, Platten M, Huber PE, Kuner T, von Deimling A, Wick W, Winkler F. (2015). Brain tumour cells interconnect to a functional and resistant network. Nature 528:93-98.


60. Nunes, D. and Kuner, T. (2015). Disinhibition of olfactory bulb granule cells accelerates odor discrimination in mice. Nature Communications, 6:8950.


61. Venkataramani, V., Herrmannsdörfer, F., Heilemann, M., Kuner, T. (2016). SuReSim – Simulating localization microscopy experiments from ground truth models. Nature Methods, in press.


62. Mohapatra, N., Tønnesen, J., Vlachos, A., Kuner, T., Deller, T., Nägerl, V.U., Santamaria, F. and Jedlicka, P. (2016). Spines slow down dendritic chloride diffusion and affect short-term ionic plasticity of GABAergic inhibition. Scientific Reports, in press.



B. Übersichtsartikel


1.   Schoepfer, R., Monyer, H., Sommer, B., Wisden, W., Sprengel, R., Kuner, T., Lomeli, H., Herb, A., Kohler, M., Burnashev, N., and et al. (1994). Molecular biology of glutamate receptors. Prog Neurobiol. 42, 353-7.


2.   Seeburg, P. H., Burnashev, N., Kohr, G., Kuner, T., Sprengel, R., and Monyer, H. (1995). The NMDA receptor channel: molecular design of a coincidence detector. Recent Prog Horm Res 50, 19-34.


3.   Seeburg, P. H., Single, F. N., Kuner, T., Higuchi, M., and Sprengel, R. (2001). Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouse. Brain Research 907, 233-243.


4.   Sprengel, R., Aronoff, R., Völkner, M., Schmitt, B., Mosbach, R., and Kuner, T. (2001). Glutamate receptor channel signatures. Trends in Pharmacological Sciences 22, 7-10.


5.   Kuner, T. #, Seeburg, P. H., and Guy, H. R. (2003). A common architecture of potassium channels and ionotropic glutamate receptors? Trends in Neurosciences 26, 27-32.


6.   Kuner, T.  and Schaefer, A. T. (2011). Moleküle, Zellen und Netzwerke für die Verarbeitung von Geruchsreizen im Riechkolben der Maus. NeuroForum 03/11, 102-109.


7.   Berglund, K., Kuner, T., Feng, G., Augustine, G.J. (2011). Imaging synaptic inhibition with the genetically encoded chloride indicator Clomeleon. CSH Protocols, doi:10.1101/pdb.prot066985.


8.   Luo, C., Kuner, T., & Kuner, R. (2014). Synaptic plasticity in pathological pain. Trends in Neurosciences, 37(6), 343–355. doi:10.1016/j.tins.2014.04.002


9.   Körber. C., & Kuner, T. (2016). Molecular machines regulating the release probability of synaptic vesicles at the active zone. Frontiers in Synaptic Neuroscience. doi: 10.3389/fnsyn.2016.00005



C. Buchkapitel


1.   Seeburg, P. H., Sprengel R.,  Monyer, H., Higuchi, M., Kuner, T., Köhr, G., Herb, A., Mosbacher, J., Jonas, P. and Burnashev, N. (1995) Glutamate Receptor Channels in CNS: Molecular and Functional Aspects. In: Puberty: Basic and Clinical Aspects - Frontiers in Endocrinology (C. Bergada and J. A. Moguilevsky, eds.) Ares Seron Symposia Publications 10, 183-191.


2.   Kuner, T., Wollmuth, L. P., and Sakmann, B. (1999). The ion conducting pore of glutamate receptor channels. In Handbook of Experimental Pharmacology. Eds Peter Jonas and Hannah Monyer. Springer Verlag, Heidelberg.


3.     Kuner, T., Tokumaru, H., and Augustine, G. J. (2002). Peptides as probes of protein-protein interactions involved in neurotransmitter release. Current Topics in Membranes, Vol 52. Eds Sidney A.Simon and Thomas J. McIntosh. Academic Press, San Diego.


4.   Kuner, T. (2009). Ultrastructural organization of release sites in the calyx of Held. In The New Encyclopedia of Neuroscience, Larry Squire, ed. (Elsevier, Oxford)


5.   Berglund, K., Kuner, T., Augustine, G.J. (2009). Clomeleon. In Physiology and Pathology of Chloride Transporters and Channels in the Nervous System: from Molecules to Diseases. F. Javier Alvarez-Leefmans and Eric Delpire, eds. (Elsevier, Oxford).



D. Bücher


1.   Kämmerer, A., Kuner, T., Maissen, T. und Wink, M. (2015) Gewalt und Altruismus: Interdisziplinäre Annäherungen an ein grundlegendes Thema des Humanen. Schriften des Marsilius-Kollegs Band 14, Universitätsverlag Winter, Heidelberg.


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