SuReSim (Super Resolution Simulation) is an open-source simulation software for Single Molecule Localization Microscopy (SMLM). The workflow of the SuReSim algorithm starts from a ground truth structure and lets the user choose to either directly simulate 3D localizations or to create simulated *.tiff-stacks that the user can analyze with any given SMLM reconstruction software.
A 3D structure of any geometry, either taken from electron microscopy, designed de-novo from assumptions or known structural facts, is fluorophore-labeled in silico. A defined set of parameters is used to calculate and visualize the 3D localizations of the corresponding labels.
The software package is accompanied with a library of model structures that can be imported and simulated.

Download and installation

SuReSim is written in Java and licensed under the terms of the GNU GPLv3 license as a standalone software package.
To execute the runnable JAR file Java Runtime Environment 8 (JRE) is required. The current version of the JRE can be downloaded for free from
For Mac users the latest version of Java did not work reliably. We recommend version 1.8.51 which worked perfectly well on all our test setups.
To install SuReSim download the latest version and place it in any directory you like. Be sure to copy the folder SuReSim_lib as well.

SuReSim Software

Model Library

User Manual

Article in Nature Methods

Letzte Änderung: Mon, 15.10.2018
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